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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in lots of tumors, which results in chromosome instability, and so may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two extra splice variants ended up described but detailed nucleotide sequence info is just not yet readily available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified via the antibody.
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This information outlines further possible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best more info final results are...
Antibodies certain to the blocking peptide not bind towards the epitope about the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which contribute on the temporal coordination of every mitotic celebration. Cyclin E sorts a posh with and features for a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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